Comparison of Immunoblotting and ELISA for Detection of Anti-Ganglioside Antibodies in Children with Guillain-Barre Syndrome
نویسندگان
چکیده مقاله:
Background: Anti-ganglioside antibody assays are widely used for diagnosis of autoimmune peripheral neuropathies. Objective: This study aimed to determine serum levels of anti-ganglioside antibodies in children with Guillain-Barre syndrome by immunoblotting technique and compare the results with those obtained by ELISA method. Method: In this investigation, 50 children with Guillain-Barre syndrome (GBS) who were admitted from July 2006 to July 2008, to Tabriz Children’s hospital in the northwest of Iran were studied. 30 children admitted for various other reasons than GBS were randomly selected as a control group. The levels of anti-ganglioside antibodies in serum were measured by ELISA and immunoblotting methods using commercial kits. Results: Anti-ganglioside antibodies (IgG) were detected in 16 (32%) GBS patients and in 1 (3.3%) control using ELISA assay. However, by employing immunoblotting technique, antibodies against seven gangliosides were found positive in 28 (56%) GBS patients and none in the control group. The sensitivities of immunoblotting and ELISA methods were 56% and 32% and their specificities were 100% and 97%, respectively (p
منابع مشابه
comparison of immunoblotting and elisa for detection of anti-ganglioside antibodies in children with guillain-barre syndrome
background: anti-ganglioside antibody assays are widely used for diagnosis of autoimmune peripheral neuropathies. objective: this study aimed to determine serum levels of anti-ganglioside antibodies in children with guillain-barre syndrome by immunoblotting technique and compare the results with those obtained by elisa method. method: in this investigation, 50 children with guillain-barre syndr...
متن کاملComparison of immunoblotting and ELISA for detection of anti-ganglioside antibodies in children with Guillain-Barre syndrome.
BACKGROUND Anti-ganglioside antibody assays are widely used for diagnosis of autoimmune peripheral neuropathies. OBJECTIVE This study aimed to determine serum levels of anti-ganglioside antibodies in children with Guillain-Barre syndrome by immunoblotting technique and compare the results with those obtained by ELISA method. METHOD In this investigation, 50 children with Guillain-Barre synd...
متن کاملDetection of anti-ganglioside antibodies in Guillain-Barre syndrome.
Sera from 40 patients with Guillain-Barré syndrome (GBS), including the subtypes acute inflammatory demyelinating polyneuropathy (AIDP), acute motor axonal neuropathy (AMAN), acute motor and sensory axonal neuropathy (AMSAN), and Miller Fisher syndrome (MFS) were examined for the presence of anti-ganglioside antibodies using the ganglioside agglutination assay, and the enzyme-linked immunosorbe...
متن کاملDetection of serum anti-ganglioside antibodies by latex agglutination assay in Guillain-Barré syndrome: comparison with ELISA.
OBJECTIVE Rapid detection of serum anti-ganglioside antibodies in Guillain-Barré syndrome (GBS) could facilitate early diagnosis and early initiation of treatment, which might shorten the term of illness and reduce sequelae. We examined serum anti-ganglioside antibodies in patients with GBS using the latex agglutination assay developed by Alaedini and Latov (J Immunoassay 21: 377-386, 2000) wit...
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عنوان ژورنال
دوره 7 شماره 2
صفحات 117- 123
تاریخ انتشار 2010-06-01
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